M-1000 Bioprocess Biochemistry Analyzer – Easy to use

M-1000 Bioprocess biochemistry analyzer is highly integrated, cost-effective, accurate and precise, it is a good tool for process analysis

M-1000 Bioprocess Biochemistry Analyzer

Precision Fermentation Analysis • Comply with the relevant GMP regulations • Multi-Parameter Testing

Core Technology
Advanced bio-oxidase membrane sensors and ion selective electrodes deliver laboratory-grade accuracy for:
1. Glucose, Lactate, Glutamine, NH₄⁺, K⁺, pH etc. (15+ parameters)
2. Real-time automatic calibration, automatic sample loading, and automatic testing
3. H₂O₂-based enzymatic quantification, good accuracy and precision, a long service life and low cost

Industry-Leading Features

Performance	Automation	Compliance
• 10-sample parallel processing	• Auto-calibration with integrated kits	• GMP regulations
• Dual-reaction cell design	• Self-cleaning electrodes	• Store and export up to 10,000 pieces of data
• CV <2% repeatability	• Intelligent dilution modes	• CE regulations
• 9 items simultaneous measurement	• Liquid level detection	• LIMS integration ready, FDA 21 CFR Part 11

M-1000 Bioprocess Biochemistry Analyzer display animation:

Test Parameters:

Glucose

Lactate

Glutamate

Lysine

Ethanol

Methanol

Glutamine

Glycerol

Xylose

Sucrose

Lactose

pH

NH4+

K+

Na+

Ca++

etc.

The Principle of M-1000 Bioprocess Biochemistry Analyzer：

Precision Bio-Oxidase Membrane Electrochemical Sensing

The test substance contained in the sample will undergo an enzymatic hydrolysis reaction under the catalysis of an immobilized bio-oxidase to generate hydrogen peroxide. The concentration of the test substance is calculated by the hydrogen peroxide concentration that is generated in the testing process of hydrogen peroxide electrode.

The device will automatically calibrate standard liquids at known concentrations, and the voltage of the standard liquid is a measure of the concentration of the test substance. The unknown concentration can be obtained through the comparison with the voltage signal of the standard liquid.

For enzyme immobilization, the following articles can be referred to:

https://www.sciencedirect.com/topics/engineering/enzyme-immobilization

M-1000 Bioprocess Biochemistry Analyzer: Advanced Monitoring Solution

Precision Biosensing • Automated Operation • Multi-Industry Applications

Bio-Oxidase Electrochemical Sensing
Proprietary immobilized enzyme membranes catalyze analyte-specific hydrolysis, generating H₂O₂ for electrochemical quantification. CV<2%

ISE Technology
Ion-selective electrodes deliver Nernstian response (slope 56±2 mV/decade) with <5% cross-sensitivity. NH₄⁺ detection limit: 10⁻⁶ M

Automated Operation

Feature	Specification	Benefit
Auto – sampling	10-position rack	90% reduction in manual handling
Auto – Calibration	NIST-traceable standards	<±5% accuracy and <2% CV over 30 days
Auto – Self-cleaning	pH-balanced buffer system	Zero point voltage determination

Key Specifications

Throughput: 10 samples/batch (4 biochemical + 5 ionic parameters)
Data Integrity: 21 CFR Part 11 compliance with audit trail
Connectivity: LIMS/SCADA via OPC-UA (RJ45/RS232/USB)
Interface: 10.1″ industrial touchscreen

Applications

Pharmaceutical: Real-time biological culture metabolism monitoring in bioreactors
Brewing: Ethanol/glucose dynamics during fermentation
Research: Microbial metabolic parameters analysis
Biofuels: Glycerol inhibition detection
Amino acid production: Lysine concentration test

Competitive Advantages

50% more convenience vs. M-900 Plus, a brand-new, smooth and durable system
Unified Platform: Biosensor + ISE integration
Zero Method Development: Pre-validated SOPs for 15+ analytes

Technical Validation:

“Achieved 98.7% correlation with HPLC in glutamine monitoring”
― Journal of Process Control, 2024

FAQ

Q: Training requirements?
A: 2-hour certification program (ISO 17025 compliant).

Q: Data export formats?
A: Export to .CSV/.PDF with metadata for FDA submissions.

Q: Maintenance protocol?
A: Automated self-diagnostics; annual sensor recalibration.

M-1000 Bioprocess Biochemistry Analyzer Technical Parameter:

Item	Test range	Precision, CV	Remark
Glucose	0.3~9 g/L	2%	Resolution 0.1g/L
Glucose	0.05~1 g/L	2%	Resolution 0.01g/L
Lactate	0.03~2 g/L	2%
Glutamate	0.03~1 g/L	2%
Glutamine	0.03~1 g/L	2%
Lysine	0.03~1 g/L	2%
Ethanol	0.03~1 g/L	2%
Methanol	0.03~0.5 g/L	2%
Glycerol	0.03~1 g/L	2%
Xylose	0.03~1 g/L	2%
Sucrose	0.03~1 g/L	2%
pH	6.50~8.00	0.01
NH4+	2.0~30.0mmol/L	2%
Na+	60.0~200.0mmol/L	2%
K+	0.5~15.0mmol/L	2%
Ca++	0.1~5.0mmol/L	2%
Optional dilution module for automatic dilution 10-30 times Test principle: Enzyme electrode, ISE method Electrode: Single replaceable electrode Resolution: 0.01g/L; 0.01mmol/L Precision (CV%): <2% Enzyme membrane life: Glucose: ≤30 days, Lactate:≤ 30 days, Glutamate: ≤30 days, Glutamine: ≤15 days, Lysine: ≤30 days, Ethanol / methanol:≤10 days, Glycerol:≤15 days, Xylose:≤15 days, NH4+ electrode: ≥6 months, Na + electrode: ≥6 months, K + electrode: ≥6 months, Ca 2+ electrode: ≥6 months, pH electrode: ≥6 months Ambient temperature: 10~35°C Relative humidity: ≤85% (no condensation water) External dimension: 313(W)×509(H)×580(L) mm Power supply: 110V or 220V, 50Hz, 60Hz, separate three-hole socket with good grounding Power: ＜100VA Weight: 38kg Screen: 10.1-inch color touch screen Techincal Link：https://www.ebiotrade.com/newsf/2025-3/20250306204209950.htm On M-1000 analyzer involves consumables, please check: http://www.siemanbio.com/product/consumables/